Teaching Students an Effective Methodology of Reproduction of Syringa Vulgaris in the Laboratory

96 189

Authors

  • G.I. Isaev Khoja Akhmet Yassawi International Kazakh-Turkish University
  • D.B. Aptarbek Khoja Akhmet Yassawi International Kazakh-Turkish University

Keywords:

Syringa vulgaris, murashige-skuga nutrient medium, microclonal reproduction, explant, callus, technique, technology, in vitro, innovations.

Abstract

This article describes the methods of identifying effective ways to study the
reproduction of the genus Syringa vulgaris in laboratory conditions in the laboratory of Akhmet Yassawi university and its introduction into the educational process at the University. At the present time, students are developing the ability to organize their practical work, demonstrating microclonal propagation of plants in the laboratory.
When conducting scientific research, morphological and control, comparative and measuring methods were used. Representatives of the siren family Syringa vulgaris were given the results of complex studies on the reproduction of valuable genotypes using biotechnological methods,
micropropagation and plant health improvement in the laboratory in vitro. It is recommended to study the results of the study in the syllabus of the discipline «Biotechnology» of the University in the amount of 1 hour.
During the study, students were formed the theoretical foundations of clonal micropropagation of plants, asexual reproduction in vitro. The optimal time for the introduction of the explant in vitro was determined. Under in vitro conditions, an effective method for disinfecting
shoots when growing lilacs with the introduction of plant explants into a nutrient medium is proposed. In the research conducted in the laboratory «Biotechnology» of Akhmet Yassawi University, 15 students of the group «ZhBI-811» in biology conducted a control of the ability to grow in the nutrient medium of Murasiga-Skuga explant lilac. The influence of genotypic
characteristics and hormonal composition of the nutrient medium on the cultivation of lilac shoots In vitro, the content of nutrient media in the root formation of lilac explants were revealed.
As a result of research work, effective methods of reproduction of microclone in general, based on the technology of reproduction of ornamental shrub lilac can be used as auxiliary tools for future specialists, students in the field of biology and the results of this work will be implemented in the content of the disciplines «Biotechnology», «Dendrology».

References

ПАЙДАЛАНЫЛҒАН ӘДЕБИЕТТЕР ТІЗІМІ

Молканова О.И., Спиридович Е.В., Коновалова Л.Н. и др. Комплексное изучение интродуцированных видов и сортов рода Syringa L. // Вестник Удмуртского университета. – 2011. – №2. – С. 66–73.

Деменко И.В. Проблемы и возможности микроклонального размножения садовых растений // Известия Тимирязевской сельскохозяйственной академии. – 2005. – Вып. 2. – С. 48–58.

Da Silva D.P.C., Ozudogru E.A., Dos Reis M.V., Lambardi M. In vitro conservation of ornamental plants // Ornamental Horticulture. – 2018. – Vol. 24(1). – P. 28–33.

Лях Е.М. Изучение сортов Syringa vulgaris из коллекции Центрального сибирского ботанического сада // Растительный мир Азиатской России. – 2015. – №3 (19). – С. 99–103.

Молканова О.И., Зинина Ю.М., Македонская Н.В., Брель Н.Г., Фоменко Т.И., Спиридович Е.В. Разработка биотехнологических приемов размножения сирени обыкновенной // Физиол. и биохим. культур растений. – 2010. – Т. 42. – №2. – С. 117–124.

Бутенко Р.Г. Культура изолированных протопластов, клеток тканей в решении задач физиологии растений // Новые направления в физиологии растений. – М.: Наука, 1985. – С. 16–32.

Da Costa C.T., de Almeida M.R., Ruedell C.M., et al. When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings // Front plant Sci. – 2013. – Vol. 4. – P. 1–19.

Rikiishi K., Matsura T., Maekawa M., Takeda K. Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos // Breeding Science. – 2008. – №58. – P. 129–135.

Чурикова О.А., Криницына А.А. Влияние спектрального состава света на укоренение сирени (Syringa vulgaris L.) в культуре in vitro // Вестник КазНу. – 2015. – №1/2 (43). – С. 607–612.

Каменский П.А., Сазонов А.Э., Федянин А.А., Садовничий В.А. Биологические коллекции: стремление к идеалу // Acta Naturae. – 2016. – Т. 8. – №2 (29). – С. 6–10.

Салыбекова Н.Н., Исаев Ғ.И., Айдарханова Б.К., Шынтемирова А.С., Сәулет С. Биологиялық білім беруде жоба жұмыстарын құру мен қолданудың мүмкіндіктері // Ясауи университетінің хабаршысы. – 2019. – №4 (114). – Б. 93–103.

Орлов П.А. Клеточные и генно-инженерные технологии модификации растений. – Минск: Тонпик, 2006. – 248 с.

Молканова О.И., Чурикова О.А., Коновалова Л.Н., Окунева И.Б. Клональное микроразмножение интродуцированных сортов Syringa vulgaris L. // Вестник Москва. – 2002. – №4. – С. 8–14.

Charlebois D., Richer C. In vitro propagation of Syringa vulgaris «Katherine Havemeyer» and «Charles Joly» // Canadian Journal of Plant Science. – 2004. – №84 (1). – P. 279–289.

Крючкова В.А. Биотехнологические приемы оптимизации микроклонального размножения и адаптации генотипов сирени (Syringa vulgaris L.): автореф. ... канд. биол. наук. – М., 2005. – 204 с.

Cui H., Gu X., Shi L. In vitro proliferation from axillary buds and ex vitro protocol for effective propagation of Syringa x hyacinthiflora «Luo Lan Zi» // SCI. HORT. – 2009. – V. 121. – P. 186–191.

Брель Н.Г., Фоменко Т.И., Спиридович Е.В. Особенности культивирования тканей рода сирень (Syringa L.) в культуре in vitro // Материалы III Международной конференции, посвященной 110-летию со дня рождения академика Н.В. Смольского (7–9 октября 2015, Минск). – Минск, 2015. – С. 250–254.

REFERENCES

Molkanova O.I., Spiridovich E.V., Konovalova L.N. i dr. Kompleksnoe izuchenie introducirovannyh vidov i sortov roda Syringa L. [Comprehensive study of introduced species and varieties of the genus Syringa L] // Vestnik Udmurtskogo universiteta. – 2011. – №2. – S. 66–73. [in Russian]

Demenko I.V. Problemy i vozmojnosti mikroklonalnogo razmnojenia sadovyh rastenii [Problems and opportunities of micro propagation of garden plants] // Izvestiia Timiriazevskoi selskohoziaistvennoi akademii. – 2005. – Vyp. 2. – S. 48–58. [in Russian]

Da Silva D.P.C., Ozudogru E.A., Dos Reis M.V., Lambardi M. In vitro conservation of ornamental plants // Ornamental Horticulture. – 2018. – Vol. 24 (1). – P. 28–33.

Liah E.M. Izuchenie sortov Syringa vulgaris iz kollekcii Centralnogo sibirskogo botanicheskogo sada[Study of varieties of Syringa vulgaris from the collection of the Central Siberian Botanical Garden] // Rastitelnyi mir Aziatskoi Rossii. – 2015. – №3 (19). – S. 99–103. [in Russian]

Molkanova O.I., Zinina Iu.M., Makedonskaia N.V., Brel N.G., Fomenko T.I., Spiridovich E.V. Rarabotka biotehnologicheskih priemov razmnozheniia sireni obyknovennoi [Development of biotechnological methods of propagation of common lilac] // Fiziol. I biohim. kultur rastenii. – 2010. – V. 42. – №2. – S. 117–124. [in Russian]

Butenko R.G. Kultura izolirovannyh protoplastov, kletok tkanei v reshenii zadach fiziologii rastenii[Culture of isolated protoplasts, tissue cells in solving problems of plant physiology] // Novye napravleniia v fiziologii rastenii. – M.: Nauka, 1985. – S. 16–32. [in Russian]

Da Costa C.T., de Almeida M.R., Ruedell C.M., et al. When stress and development go hand in hand: main hormonal controls of adventitious rooting in cuttings // Front plant Sci. – 2013. – Vol .4. – P. 1–19.

Rikiishi K., Matsura T., Maekawa M., Takeda K. Light control of shoot regeneration in callus cultures derived from barley (Hordeum vulgare L.) immature embryos // Breeding Science. – 2008. – №58. – P. 129–135.

Churikova O.A., Krinicyna A.A. Vlijanie spektralnogo sostava sveta na ukorenenie sireni (Syringa vulgaris L.) v kulture in vitro [The influence of the spectral composition of light on the rooting of lilacs] // Vestnik KazNu. – 2015. – №1/2 (43). – S. 607–612. [in Russian]

Kamenski P.A., Sazonov A.Ie., Fedianin A.A., Sadovnichi V.A. Biologicheskie kollekcii: stremlenie k idealu [Biological collections: striving for the ideal] // Acta Naturae. – 2016. – V.8. – №2 (29). – S. 6–10. [in Russian]

Salybekova N.N., Isaev G.I., Aidarhanova B.K., Shyntemirova A.S., Saulet S. Biologiialyq bіlіm berude joba jumystaryn quru men qoldanudyn mumkіndіkterі [Possibilities of creating and using project work in biological education] // Iasaui universitetіnіn habarshysy. – 2019. – №4 (114). – B. 93–103. [in Kazakh]

Orlov P.A. Kletochnyei genno-injenernye tehnologii modifikacii rastenii [Cellular and genetic engineering technologies for plant modification]. – Minsk: Tonpik, 2006. – 248 s. [in Russian]

Molkanova O.I., Churikova O.A., Konovalova L.N., Okuneva I.B. Klonal'noe mikrorazmnozhenie introducirovannyh sortov Syringa vulgaris L. [Clonal micropropagation of introduced varieties of Syringa vulgaris L] // Vestnik Moskva. – 2002. – №4. – S. 8–14. [in Russian]

Charlebois D., Richer C. In vitro propagation of Syringa vulgaris «Katherine Havemeyer» and «Charles Joly» // Canadian Journal of Plant Science. – 2004. – №84 (1). – P. 279–289.

Kriuchkova V.A. Biotehnologicheskie priemy optimizacii mikroklonalnogo razmnojeniia i adaptacii genotipov sireni [Biotechnological techniques for optimizing micropropagation and adaptation of lilac genotypes] (Syringa vulgaris L.): avtoref. ... kand. biol. nauk. – M., 2005. – 204 s. [in Russian]

Cui H., Gu X., Shi L. In vitro proliferation from axillary buds and ex vitro protocol for effective propagation of Syringa x hyacinthiflora «Luo Lan Zi» // SCI. HORT. – 2009. – V. 121. – P. 186–191.

Brel N.G., Fomenko T.I., Spiridovich E.V. Osobennosti kultivirovaniia tkanei roda siren (Syringa L.) v kulture in vitro [Features of the cultivation of tissues of the genus lilac (Syringa L.) in in vitro culture]// Materialy III Mejdunarodnoi konferencii, posviashhennoi 110-letiiu so dnia rojdeniia akademika N.V. Smolskogo (7–9 oktiabria 2015, Minsk). – Minsk, 2015. – S. 250–254. [in Russian]

Published

2022-12-29

Issue

Section

PEDAGOGY AND METHODS OF TEACHING